Cell Biology
Naeimeh Dehghani; Hamed Daneshpazhouh
Volume 1, Issue 1 , March 2025, , Pages 45-54
Abstract
Background: This study aimed to investigate the effect of docetaxel on the expression level of mitochondria- related genes including mitochondrial transcription A (TFAM) and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-COX 1) in mouse MII oocytes following vitrification by cryotop.Methods: ...
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Background: This study aimed to investigate the effect of docetaxel on the expression level of mitochondria- related genes including mitochondrial transcription A (TFAM) and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-COX 1) in mouse MII oocytes following vitrification by cryotop.Methods: Oocytes were selected by simple random sampling and distributed amongst five experimental groups (control (n=126), docetaxel (n=132), docetaxel + cryoprotectant agent (CPA) (n=134), docetaxel+ Vitrification (n=132), and vitrification (n=123)). In this experimental study, the survival and fertilization of methaphase II (MII) mouse oocytes were assessed after vitrification by cryotop. In the second experiment, the effects of docetaxel on the expression of TFAM and COX1 genes were determined in vitrified-warmed oocytes by real-time RT-PCR. Each experimental group was compared with the control group.Results: The results showed a significant reduction in the survival rate of each group in comparison with the controls (P<0.05). The survival rate was significantly lower in both vitrification groups (Docetaxel+ vitrification, vitrification) than in non-vitrification groups (fresh control and Docetaxel) (P<0.05). The survival rate of vitrified/warmed oocytes was significantly higher in the Docetaxel+ vitrification group compared with the vitrification group (P=0.005). There were significant differences in the fertilization rate between the docetaxel group with the other groups (P<0.05). There were significant differences between the expression level of Cox1 gene in the control group with docetaxel and vitrification groups (P<0.05). the results showed that the expression level of COX1, TFAM genes was significantly high in CPA, vitrification group (P<0.05) and pretreatment of oocytes with docetaxel can decrease the expression level of cox1, TFAM genes in the vitrified group (vitrification) and non-vitrified group (CPA) (P<0.05).Conclusions: This study indicates that vitrification of mouse MII oocytes can lead to an increase in mitochondrial gene expressions (specifically TFAM and COX1). However, the pretreatment of oocytes with docetaxel is observed to result in a decrease in mitochondrial gene expression.
