Nanobiotechnology
Foozieh Moghadami; Mahdi Kalantari; Fatemeh Hajmoradi
Volume 1, Issue 1 , March 2025, , Pages 1-10
Abstract
Background: The membrane-bound sorbitol dehydrogenase is a member of the flavoprotein dehydrogenase–cytochrome complex located in the respiratory chain of the genus of Gluconobacter oxidizes D-sorbitol to L-sorbose, the vitamin C intermediate production, with high specificity. In this research, ...
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Background: The membrane-bound sorbitol dehydrogenase is a member of the flavoprotein dehydrogenase–cytochrome complex located in the respiratory chain of the genus of Gluconobacter oxidizes D-sorbitol to L-sorbose, the vitamin C intermediate production, with high specificity. In this research, the silver nanoparticles effect on L-sorbose production and the sorbitol dehydrogenase activity by Gluconobacter oxydans were investigated through response surface methodology.Methods: The silver nanoparticles effect on L-sorbose production was studied in a 2.5 L laboratory-scale bioreactor. The central composite design was employed for evaluation of the silver nanoparticles effect on sorbitol dehydrogenase activity at different pH and temperatures. The sorbitol dehydrogenase of Gluconobacter oxydans activity was evaluated in the membrane fractions by colorimetric method.Results: The results showed that the addition of 50 mg/L of silver nanoparticles into the culture medium caused a decrease of 2.3 and 1.7 times in L-sorbose production and dry cell weight, respectively. Studying the sorbitol dehydrogenase activity through response surface methodology showed that the highest and lowest activity were observed when 0 and 100 mg/L of silver nanoparticles were added into the culture medium, respectively (35 and 1.5 U/L). The temperature and pH showed a direct effect on the sorbitol dehydrogenase. The effects of the three parameters of temperature, pH, and nanoparticle concentration were linear. The parameters of temperature and silver nanoparticles concentrations showed a positive interaction.Conclusion: It could be concluded that silver nanoparticles decreased the L-sorbose production by Gluconobacter oxydans through inhibiting the membrane-bound sorbitol dehydrogenase activity and cell growth.
